Department or Program

Biological Chemistry

Abstract

The spatial arrangement of RNA localization plays a role in the expression and turnover of transcripts in B. burgdorferi, including those that are involved in its virulence and pathogenicity. RNA localization was experimented with by using techniques such as fluorescence in situ hybridization with RNA polymerase subunits and regulatory Factors, using RNA aptamers through induction, and imaging using stimulated emission depletion nanoscopy. Throughout experimentation, it will be determined whether RNA transcripts distribute themselves along the full length of B. burgdorferi cells or localize at the poles, septa of dividing cells, or are concentrated at other foci within the cells. In both fluorescence in situ hybridization and RNA aptamers a fluorescence signal was observed.

Level of Access

Restricted: Archival Copy [No Access]

First Advisor

Schlax, Paula

Date of Graduation

5-2021

Degree Name

Bachelor of Science

Number of Pages

35

Embargoed

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