Event Website
http://www.bates.edu/mt-david-summit.xml
Start Date
1-4-2011 1:45 PM
End Date
1-4-2011 3:00 PM
Description
The genetic material of eukaryotic cells is contained in the nucleus, where the processes of DNA replication and transcription are highly regulated. Because proteins are produced in the cell's cytoplasm, those necessary for nuclear activities must be transported into the nucleus. A nuclear localization signal (NLS) is the portion of a protein's amino acid sequence that acts as a "tag," allowing interactions with necessary accessory proteins. Ribosomal S6 kinases (RSKs) are ubiquitous signalers that regulate genes critical to development, and aberrant RSK expression has appeared in prostate and breast cancer tumors, late-onset diabetes, and mental retardation (Anjum and Blenis 2008, Carriere et al. 2008). Since RSK3 is involved with cell cycle regulation, it offers promise as a potential chemotherapeutic target. This study will probe various portions of the human RSK3 sequence for ability to transport a reporter protein to the cell nucleus, with the aim of identifying a functional NLS.
Download
Characterizing a Nuclear Localization Signal in RSK3
The genetic material of eukaryotic cells is contained in the nucleus, where the processes of DNA replication and transcription are highly regulated. Because proteins are produced in the cell's cytoplasm, those necessary for nuclear activities must be transported into the nucleus. A nuclear localization signal (NLS) is the portion of a protein's amino acid sequence that acts as a "tag," allowing interactions with necessary accessory proteins. Ribosomal S6 kinases (RSKs) are ubiquitous signalers that regulate genes critical to development, and aberrant RSK expression has appeared in prostate and breast cancer tumors, late-onset diabetes, and mental retardation (Anjum and Blenis 2008, Carriere et al. 2008). Since RSK3 is involved with cell cycle regulation, it offers promise as a potential chemotherapeutic target. This study will probe various portions of the human RSK3 sequence for ability to transport a reporter protein to the cell nucleus, with the aim of identifying a functional NLS.
http://scarab.bates.edu/mt_david_summit/MDS2011/02Poster/11