Event Website
http://www.bates.edu/mt-david-summit.xml
Start Date
1-4-2011 1:45 PM
End Date
1-4-2011 3:00 PM
Description
RpoS is an alternate sigma factor that regulates the expression of a number of key virulence genes in Borrelia burgdorferi, the spirochete that causes Lyme Disease. The transcriptional upregulation of this gene is a function of the changes in temperature, pH and cell density that occur immediately preceding and during the bacteria's transition from the Ixodes vector to the mammalian host. In addition to these well-characterized transcription factors, recent studies have suggested the involvement of small non-coding RNAs (sRNAs) in the translational regulation of rpoS, specifically the sRNA DsrABb. This study seeks to characterize the interaction of DsrABb with the 5' untranslated region of rpoS through mutational studies and affinity assays.
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Mutational Studies of RpoS and Translational Initiation in Borrelia burgdorferi
RpoS is an alternate sigma factor that regulates the expression of a number of key virulence genes in Borrelia burgdorferi, the spirochete that causes Lyme Disease. The transcriptional upregulation of this gene is a function of the changes in temperature, pH and cell density that occur immediately preceding and during the bacteria's transition from the Ixodes vector to the mammalian host. In addition to these well-characterized transcription factors, recent studies have suggested the involvement of small non-coding RNAs (sRNAs) in the translational regulation of rpoS, specifically the sRNA DsrABb. This study seeks to characterize the interaction of DsrABb with the 5' untranslated region of rpoS through mutational studies and affinity assays.
http://scarab.bates.edu/mt_david_summit/MDS2011/02Poster/3