Event Website
http://www.bates.edu/mt-david-summit.xml
Start Date
1-4-2011 1:45 PM
End Date
1-4-2011 3:00 PM
Description
The bacterium Aggregatibacter actinomycetemcomitans is a gram-negative periodontopathogen associated with localized aggressive periodontitis as well as several systemic infections such as endocarditis, and cardiovascular disease. In the oral cavity, A. actinomycetemcomitans must adapt to an iron-limited environment in order to remain attached to the tooth and other oral surfaces by regulating gene expression of several biofilm determinant factors including fimbriae, extracellular polymeric substance, and lipopolysaccharide. The objectives of this study are to determine the proper working conditions and parameters for isolating total RNA from A. actinomycetemcomitans to establish a complete transcriptional profile using a microarray analysis, as well as investigate the role of iron-regulated sRNA molecules in biofilm formation using microtitre biofilm assays. Collectively, this data will be used to confirm prior real-time PCR data on select biofilm determinant genes. This research will lead to a better understanding of how A. actinomycetemcomitans modulates its gene expression in the oral cavity, and has clinical implications for disease susceptibility and patterns of occurrence.
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Regulation of Global Gene Expression and Biofilm Formation in Response to Iron Limitation by Aggregatibacter actinomycetemcomitans
The bacterium Aggregatibacter actinomycetemcomitans is a gram-negative periodontopathogen associated with localized aggressive periodontitis as well as several systemic infections such as endocarditis, and cardiovascular disease. In the oral cavity, A. actinomycetemcomitans must adapt to an iron-limited environment in order to remain attached to the tooth and other oral surfaces by regulating gene expression of several biofilm determinant factors including fimbriae, extracellular polymeric substance, and lipopolysaccharide. The objectives of this study are to determine the proper working conditions and parameters for isolating total RNA from A. actinomycetemcomitans to establish a complete transcriptional profile using a microarray analysis, as well as investigate the role of iron-regulated sRNA molecules in biofilm formation using microtitre biofilm assays. Collectively, this data will be used to confirm prior real-time PCR data on select biofilm determinant genes. This research will lead to a better understanding of how A. actinomycetemcomitans modulates its gene expression in the oral cavity, and has clinical implications for disease susceptibility and patterns of occurrence.
http://scarab.bates.edu/mt_david_summit/MDS2011/02Poster/30