Event Website
http://www.bates.edu/mt-david-summit.xml
Start Date
1-4-2011 1:45 PM
End Date
1-4-2011 3:00 PM
Description
Human periodontal diseases are infectious, cause chronic inflammation and tooth loss, and are correlated with instances of osteoporosis. Studies suggest that genetic predisposition contributes to the severity of bone loss and progression of the disease. Previous studies have shown that the mouse strain BALB/cByJ is hereditarily susceptible to alveolar bone loss with infection with Porphyromonas gingivalis, and that gene expression changes with infection. In this study we sought to distinguish the genes that may confer susceptibility to bone loss associated with periodontal disease by characterizing the expression of various bone remodeling genes that occurred in the strain. We compared the relative gingival RNA expression of sham-infected and infected BALB/cByJ mice by isolating the gingival RNA from 10 sham-infected and 10 infected mice euthanized 1, 2, and 3 weeks after infection with P. gingivalis. cDNA was made and amplified using primers associated with osteoporosis for quantization by qPCR. The results suggest several genes that may link periodontal disease and osteoporosis.
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Genetics of Susceptibility to Bone Loss Related to Periodontal Disease
Human periodontal diseases are infectious, cause chronic inflammation and tooth loss, and are correlated with instances of osteoporosis. Studies suggest that genetic predisposition contributes to the severity of bone loss and progression of the disease. Previous studies have shown that the mouse strain BALB/cByJ is hereditarily susceptible to alveolar bone loss with infection with Porphyromonas gingivalis, and that gene expression changes with infection. In this study we sought to distinguish the genes that may confer susceptibility to bone loss associated with periodontal disease by characterizing the expression of various bone remodeling genes that occurred in the strain. We compared the relative gingival RNA expression of sham-infected and infected BALB/cByJ mice by isolating the gingival RNA from 10 sham-infected and 10 infected mice euthanized 1, 2, and 3 weeks after infection with P. gingivalis. cDNA was made and amplified using primers associated with osteoporosis for quantization by qPCR. The results suggest several genes that may link periodontal disease and osteoporosis.
http://scarab.bates.edu/mt_david_summit/MDS2011/02Poster/21